Phosphofructokinase

Phosphofructokinase (PFK) is the most important regulatory enzyme of glycolysis. It is an allosteric enzyme made of 4 subunits and controlled by several activators and inhibitors. This leads to a precise control of glucose and the other monosaccharides galactose and fructose going down the glycolysis pathway.

PFK converts fructose 6-phosphate and ATP to fructose 1,6-bisphosphate and ADP. The enzyme has two sites with different affinities for ATP which is both a substrate and an inhibitor.

See also:

• PFK2 (converts fructose 6-phosphate to fructose 2,6-bisphosphate)
• PFP (reversibly interconverts fructose 6-phosphate and fructose 1,6-bisphosphate using inorganic pyrophosphate rather than ATP)
• fructose bisphosphatase (hydrolyses fructose 1,6-bisphosphate to fructose 6-phosphate)